[1]黄巍,等.香旱芹精油对金黄色葡萄球菌抑菌机理的研究[J].中国调味品,2017,(12):7-11.
 HUANG Wei,WANG Jian-qing,ZHANG Qian.Study on the Antibacterial Mechanism of Ajowan Essential Oil Against Staphylococcus aureus[J].CHINA CONDIMENT,2017,(12):7-11.
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香旱芹精油对金黄色葡萄球菌抑菌机理的研究()
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《中国调味品》[ISSN:1000-9973/CN:23-1299/TS]

卷:
期数:
2017年12期
页码:
7-11
栏目:
出版日期:
2017-12-01

文章信息/Info

Title:
Study on the Antibacterial Mechanism of Ajowan Essential Oil Against Staphylococcus aureus
作者:
黄巍1 2王建清1张倩1
(1.天津科技大学中国轻工业食品包装材料与技术重点实验室,天津 300222 ;2. 中国包装科研测试中心,天津 300457 )
Author(s):
HUANG Wei 12 WANG Jian-qing 1 ZHANG Qian 1
(1.Tianjin University of Science and TechnologyChina Light Industry Key Laboratory of Food Packaging Materials and Technology, Tianjin 300222,China; 2.China Packaging Research & Test Center, Tianjin 300457, China)
关键词:
香旱芹精油微波辅助水蒸气蒸馏GC-MS分析金黄色葡萄球菌抑菌机理
Keywords:
Ajowan essential oil microwave assisted steam distillation GC-MS analysis Staphylococcus aureus antibacterial mechanism
分类号:
TS201.3
文献标志码:
A
摘要:
采用微波辅助水蒸气蒸馏提取出香旱芹精油,对其成分进行GC-MS分析,通过测试其对金黄色葡萄球菌的抑菌圈直径、最低抑菌浓度( MIC) 和最低杀菌浓度( MBC) 评价其抑菌效果。分别从菌体微观形态,细胞溶出物、蛋白质和钾离子泄漏等方面研究香旱芹精油对金黄色葡萄球菌的抑菌机理。实验结果表明香旱芹精油的主要成分为百里香酚(45.85%),对伞花烃(20.72%)和γ-松油烯(23.57%),其对金黄色葡萄球菌的抑菌圈直径为75mm,MIC为0.25μL/mL,MBC为0.5μL/mL。香旱芹精油可使金黄色葡萄球菌细胞膜受到损伤,使细胞膜表面出现塌陷和破裂,并引起菌体细胞溶出物、蛋白质、以及钾离子的泄露,从而导致了菌体的死亡。
Abstract:
Ajowan essential oil is extracted by microwave assisted steam distillation, and its chemical composition is analyzed by GC-MS. The inhibitory diameter, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ajowan essential oil against Staphylococcus aureus are tested by agar diffusion method and dilution method.The antibacterial mechanism of ajowan essential oil against S.aureus is studied by exploring the scanning electron microscopy (SEM) image of S.aureus, the leakage of cell constituents, protein and potassium ion from S.aureus cell after suffering the treated with the ajowan essential oil. The results show that the main components of ajowan essential oil are thymol (45.85%) , p-cymene ( 20.72%),and γ-Terpinene(23.57%), which have a strong inhibitory effect on S. aureus, the diameter of the inhibition zone is 75 mm, the MIC is 0.25 μL/mL and the MBC is 0.5 μL/mL. Ajowan essential oil can destroy the cell membrane of S. aureus, and its cell membrane surface appeared folds and local collapse, resulting in a leakage of cell constituents, protein, and potassium ion from cell, leading to the death of bacteria

参考文献/References:

[1] Juliany R Calo, Philip G Crandall, Corliss A O’Bryan, et al. Essential oils as antimicrobials in food systems—a review[J]. Food Control, 2015; 54: 111-119.

[2] Pesavento G, Calonico C, Bilia A R, et al.Antibacterial activity of Oregano, Rosmarinus and Thymus essential oils against Staphylococcus aureus and Listeria monocytogenes in beef meatballs[J]. Food Control, 2015;54: 188-199.

[3]柴向华,董艳,吴克刚,等. 植物精油对食品中常见有害微生物的抑菌活性研究[J]. 现代食品科技,2016(8):123-127.

[4] Akash Kedia, Bhanu Prakash, Prashant Mishra, et al.Trachyspermum ammi L. essential oil as plant based preservative in food system[J]. Industrial Crops and Products, 2015; 69:104–109.

[5]张赟彬,刘笑宇,姜萍萍,等. 肉桂醛对大肠杆菌和金黄色葡萄球菌的抑菌作用及抑菌机理研究[J]. 现代食品科技,2015(5):31-35.

[6]丁华,王建清,王玉峰,等. 罗勒精油成分分析及抑菌性研究[J]. 中国调味品,2017,42(2):43-48.

[7] Han C, Wang J, Li Y, et al. In vitro antimicrobial activity and effect on E. coli integrity of cinnamon essential oil and rhubarb ethanol extract[J]. Food Sci Technol Res 2013; 19:1155-1163.

[8] Turgis M, Han J, Caillet S, et al. Antimicrobial activity of mustard essential oil against Escherichia coli O157:H7and Salmonella typhi[J]. Food Control 2009; 20:1073-1079.

[9] Mengying He, Ting Wu, Siyi Pan,et al.Antimicrobial mechanism of flavonoids against Escherichia coli ATCC25922 by model membrane study[J]. Applied Surface Science 2014; 305: 515-521.

[10] Jianyin Miao, Guo Liu, Chang Ke, et al. Inhibitory effects of a novel antimicrobial peptide from kefir against Escherichia coli[J]. Food Control 2016; 65: 63-72.

[11] Oroojalian F, Kasra-Kermanshahi R, Azizi M, et al. Phytochemical composition of the essential oils from three Apiaceae species and their antibacterial effects on food-borne pathogens[J].Food Chemistry, 2010, 120(3): 765-770.

[12] Sharifzadeh A, Khosravi A R, Shokri H, et al. Antifungal effect of Trachyspermum ammi against susceptible and fluconazole -resistant strains of Candida albicans[J]. J. Mycol. Med., 2015, 25(2): 143-150.

[13]Rota M C, Herrera A, Mart′nez R M, et al. Antimicrobial activity and chemical composition of Thymus vulgaris,Thymuszygis and Thymus hyemalis essential oils[J]. Food Control,2008, 19(7):681-687.

[14] Ce Shi, Xiaowei Zhang, Xingchen Zhao, et al.Synergistic interactions of nisin in combination with cinnamaldehyde against Staphylococcus aureus in pasteurized milk[J].Food Control,2017,71: 10-16.

[15] Roya Moghimi, LidaGhaderi, HasanRafati, et al. Superior antibacterial activity of nanoemulsion of Thymus daenensis essential oil against E. Coli[J].Food Chemistry 2016; 19: 410-415.

[16] Suxia Shen, Tiehua Zhang, Yuan Yuan, et al. Effects of cinnamaldehyde on Escherichia coli and Staphylococcus aureus membrane[J]. Food Control,2013,32:582-590.

[17] Ajiboye T O, Mohammed A O, Bello S A, et al. Antibacterial activity of Syzygium aromaticum seed: studies on oxidative stress biomarkers and membrane permeability[J]. Microbial Pathogenesis, 2016, 95:208-215.

[18] 刘笑宇.肉桂精油的抑菌作用、抑菌机理及其在酱牛肉防腐中的应用[D]. 上海:上海应用技术学院, 2015.

[19] Vivek Bajpai, Ajay Sharma, Kwang-Hyun Baek. Antibacterial mode of action of Cudrania tricuspidata fruit essential oil, affecting membrane permeability and surface characteristics of food-borne pathogens[J]. Food Control,2013, 32(2): 582-590.

更新日期/Last Update: 2017-12-04